PolyICSFlow: Identifying the Frequency of Polyfunctional Antigen-Specific T cells in ICS Flow Cytometry Data1 months ago
1) Introduction | 1.1) Comparison with existing tools | 1.2) Example data | 2) Installation | 3) Preparing the data | 3.1) Data pre-processing | 3.2) Cytokine gating | 3.3) Defining cell populations | 4) Using polyICSFlow | 4.1) Assigning cytokine positivity | 4.2) Assigning marker combinations | 4.3) Calculating peptide-specificity | 4.3.1) Example 1: Quantifying number of functions | 4.3.2) Example 2: Quantifying individual marker combinations | 4.3.3) Example 3: Quantifying polyfunctional IFN+ responses | 5) Plotting options | 6) Session info